In as post by Since joining Crystal Mackall lab, Hyatt Balke-Want wanted to develop a robust viral-free platform that would yield enough CAR-T cells to treat patients. Under the expert eye of Steven Feldman he developed HITI, a system based on nanoplasmid DNA to specifically knock-in CAR via homology-independent targeted insertion, together with a selection marker. Upon enrichment of the engineered population, he achieved ~80% pure CAR-T cells at doses therapeutically relevant and without any evidence of off-target genomic toxicity.
A cheaper and effective approach to clinical CAR-T manufacturing!”
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